Workshop 1

Advion Interchim Scientific, 211 bis avenue JF Kennedy, BP1140, 03103 Montluçon, France

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Liquid Chromatography is a separation technique. It can be dedicated to identify and quantify compounds present in a mixture (analytical mode). Very attractive and popular technique, when the goal is to get isolated pure product from a more or less complex mixture is a flash / preparative liquid chromatography.

There are many different purification techniques like distillation, crystallization, filtration and chromatography. They all have a goal to purify and recover samples. Purification by liquid chromatography is always a challenge and there is often a compromise to obtain desired purity, loading and throughput. To improve efficiency in delivering pure compounds, chemists may balance between purity, run time and environmental considerations. This delicate balance is often necessary for both crude and final purification.

In the workshop we will cover principles of successful purification:

• Tools for method development and method transfer from TLC or HPLC
• Parameters to consider to make a successful TLC plate
• How to choose the right size and phase of the column for the efficient separation
• Parameters for optimal injection
• Practical demonstration on a puriFlash 5.250
• Q&A part