Irena Maria Choma, Hanna Nikolaichuk, Ewelina Sobstyl
Faculty of Chemistry, Maria Curie-Skłodowska University, Maria Curie-Skłodowska Sq.3, 20 031 Lublin, Poland
This email address is being protected from spambots. You need JavaScript enabled to view it.
The plant kingdom is an inexhaustible source of biologically active compounds used as components of supplements and pharmaceutical preparations. Effect directed detection (EDD), i.e. thin-layer chromatography combined with direct bioautography (TLC-DB), and TLC-screening are convenient tools for bio-profiling of active constituents of plants. In TLC-DB, the effect observed and measured directly on a TLC plate results from an action of biologically active constituents of a sample, e.g., inhibition of bacteria growth related to antibacterial activity of separated plant constituents. The detected bioactive components can be further identified using spectroscopic techniques such as GC-MS, LC-MS, IR, NMR. TLC–bio-profiling can also be used to distinguish among plants from various species and origin, find the most active ones, and point to possible adulterations. We focused on Schisandraceae family and Rhodiola rosea L. (various species, supplements, and pharmaceutical preparations) [1-3]. The most interesting findings were the strong antibacterial properties of S. chinensis and S. rubriflora fruits, as well as acetylcholinesterase, glucosidase, tyrosinase, and lipase inhibition of all tested species. Strong glucosidase inhibition was observed for Kadsura japonica fruits, which generally did not reveal biological activities. NP-PEG assay and TLC-DPPH test showed that leaves of various Schisandra species are rich in polyphenolic compounds and have strong antioxidant properties (especially leaves of S. rubriflora and K. japonica). Strong antioxidant, antibacterial and enzyme-inhibiting properties were found for the root and rhizome of R. rosea L.. Various R.rosea pharmaceutical preparations and supplements differ in their biological properties as well as in the content of marker compounds: rosavin, salidroside, and tyrosol.
References
[1] E. Sobstyl, A. Szopa, H. Ekiert, S. Gnat, R. Typek, I.M. Choma, J. Chromatogr. A, 1618 (2020) 460942.
[2] H. Nikolaichuk, M. Studziński, I.M. Choma, J. Liq. Chromatogr. Rel. Technol., 43 (2020) 361-366.
[3] H. Nikolaichuk, R. Typek, S. Gnat, M. Studziński, I.M. Choma, J. Chromatogr. A, 1649 (2021) 462217.
