Vicente Cebolla1, Carmen Jarne1, María Sancho-Albero2, Luis Membrado1, Jesús Vela3, María Savirón4, José-Manuel Escuín1, Javier Galbán5, Pilar Martín-Duque6, Jesús Santamaría2
1Instituto de Carboquímica/CSIC, Miguel Luesma Castán 4, 50018, Zaragoza, Spain
2Aragon Institute of Nanoscience/Universidad de Zaragoza, Campus Rio Ebro, 50018, Zaragoza, Spain
3Analytical Chemistry Department/Universidad de Zaragoza, Campus Río Ebro, 50018, Zaragoza, Spain
4CEQMA/CSIC-Universidad de Zaragoza, Campus San Francisco, 50009, Zaragoza, Spain
5Analytical Chemistry Department/Universidad de Zaragoza, Campus San Francisco, 50009, Zaragoza, Spain
6Instituto Aragonés de Ciencias de la Salud/Fundación ARAID, Campus San Francisco, 50009, Zaragoza, Spain
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The current scientific paradigm to understand the role of lipids in human diseases is based on the hypothesis that some diseases have distinctive lipid profiles (biomarkers) with respect to the respective healthy status.
HPTLC separation of sample, followed by densitometry and MS coupling provides a simple but powerful approach for determining lipid classes and obtaining a detailed structural elucidation of lipids present in biological extracts, allowing their exact recognition by their m/z, and confirmation by their collision-induced dissociation MS/MS data [1].
The application of HPTLC to identification in human plasma of 19 molecular species related to globotriaosylceramides (Gb3) which are considered biomarkers of Fabry disease, a lysosomal storage disorder, will be first discussed [2].
Another example is the open question of whether exosome lipids can be considered as potential cancer biomarkers. This faces our current limited knowledge of their composition. A procedure to isolate exosomes and obtain a phospholipid (PL)-class determination and identification of their molecular species will be also discussed, from embryonic murine fibroblasts (NIH-3T3 cell line), and metastatic murine skin melanoma cells with different degrees of malignancy [3].
For both examples, a detailed structural characterization of molecular species of each concerned lipid class was performed directly from the chromatographic plate, thanks to an elution-based interface, using ion-trap technology by different approaches that involve simultaneous positive and negative ESI-MS, MS/MS, or APCI.
References
[1] V.L. Cebolla, C. Jarne, J. Vela, R. Garriga, L. Membrado, J. Galbán, J. Liq. Chromatogr. & Rel. Technol. 44 (2021) 148-170.
[2] C. Jarne, L. Membrado, M. Savirón, J. Vela, J. Orduna, R. Garriga, J. Galbán, V.L. Cebolla, J. Chromatogr. A 1638 (2021), 461895.
[3] M. Sancho-Albero, C. Jarne, M. Savirón, P. Martín-Duque, L. Membrado, V.L. Cebolla, J. Santamaría, Int. J. Mol. Sci. 2021, in press.
Acknowledgements
This work was supported by DGA-FEDER (E25_20R, N&SB), AES PI21/00036, CSIC 202180E076, ISCIII PI19/01007, ERC Advanced Grant CADENCE (grant no. ERC-2016-ADG-742684)
